In the current study, we evaluate the utility of using the variation at 14 microsatellite loci and one MHC locus for lake-specific identification of sockeye salmon over its natural range. This evaluation is conducted by examining the accuracy and precision of estimated stock compositions through an analysis of simulated mixtures and samples from fisheries in coastal British Columbia. Mixtures were resolved using a 299-population baseline incorporating populations from Japan, Russia, Alaska, British Columbia, and Washington. We demonstrate that sufficient population allele frequency variation exists at microsatellite and MHC loci in sockeye salmon to enable a highly accurate identification of individual fish to lake of origin on a Pacific Rim basis.